Please use this identifier to cite or link to this item: http://repository.uinjkt.ac.id/dspace/handle/123456789/48888
Title: Test of the Immunomodulatory Effect and Measurement of Changing Acid Phosphatase Enzyme of a Water Extract Mixture of Piper betle L, (Uncaria gambir, Roxb and calcium Hydroxide on Mice Macrophage cells
Other Titles: International Seminar on Natural Products 2010 From Nature to Medicine Through Suistainable Education, R & D and Practice
Authors: Muhammad Yanis Musdja
Keywords: International Conference on Natural Products 2010;Pharmacy Seminar
Issue Date: 11-Dec-2010
Publisher: School of Pharmaceutical Sciences, Universiti Sains Malaysia
Series/Report no.: Universiti Sains Malaysia;Abstract A4-108
Abstract: Test of the Immunomodulatory Effect and Measurement of Changing Acid Phosphatase Enzyme of a Water Extract Mixture of Piper betle L, (Uncaria gambir, Roxb and calcium Hydroxide on Mice Macrophage cells Introduction: The main components of betel quid chewing which are betel leaf (Piper betle L.), gambir (Uncaria gambir Roxb) and slaked lime (calcium hydroxide) have been studied extensively but separately, thus studies on these components in combination particularly as immunomodulatory agents is scarce. 0bjectives: To ascertain the efficacy of the quid chewing components as immunomodulatory agents. Materials and Methods: A water extraction was carried out consisting a mixture of betel leaves, gambir and slaked lime, The extract was given to 7 groups of mice for 14 days with doses of 100, 200 and 400 mg/kg body weight. Two kinds of immunomodulatory drugs in syrup form commonly used in the treatment of infections were used, as positive controls. Then the peritoneal fluid of mice containing macrophage cells was isolated by performing surgery. This study calculated the number of activity and capacity of macrophage cells of mice after swallowing Staphylococcus epidermidis and also calculated changes to acid phosphatase enzyme in macrophage cells, on each group of mice. Results: The quid chewing component mixture group compared with negative controls showed an increase of phagocytic activity of 86.6%, 94.2% and 88.3% for doses of 100, 200 and 400 mg/kg body weight, respectively. Meanwhile, the phagocytic capacity obtained for an average of 50 macrophage cells was 626.1, 806.4 and 754.5 bacteria for a dose of 100, 200 and 400 mg/kg body weight, respectively. Conclusion: For the 3 doses administered, the 200 mg/kg dose showed the most efficacy where both the phagocytic activity and capacity calculated was statistically significant (p < 0.05), and changes for acid phosphatase was found statistically significant (p < 0.05) as well.
Description: To ascertain the efficacy of the quid chewing components as immunomodulatory agents
URI: http://repository.uinjkt.ac.id/dspace/handle/123456789/48888
ISBN: 978-983-44596-5-9
Appears in Collections:Prosiding Workshop/Lokakarya/Seminar



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