Please use this identifier to cite or link to this item: http://repository.uinjkt.ac.id/dspace/handle/123456789/48778
Title: Comparison of Activity and Inhibitory Mencanism between (+)Catechin and Water Extract of Gambier (Uncaria gambir Roxb) against gram positive
Other Titles: 5th Conference of AASP ( ASIAN ASSOCIATION OF SCHOOLS OF PHARMACY),
Authors: Muhammad Yanis Musdja
Amir Syarif
Ernie Hernawati P
Andria Agusta
Keywords: International Seminar;Pharmacy
Issue Date: 19-Jun-2011
Publisher: Asian Association of School of Pharmacy and School of Pharmacy ITB
Series/Report no.: 5th Conference Asian Association of School of Pharmacy;P085
Abstract: Gambir (Uncaria gambir Roxb) has since long been used by many people in the Asian region traditionally as as anti-microbial, anti diarrheal, and agent for treatment of burns. Gambier is also one component for chewing medicine to strengthen teeth. This research has the objective of comparing the antibacterial activity as well as the mechanism of inhibition between (+)-catechin and gambier extract as the basis for choosing a substance to be developed as antimicrobial drug. (+)-catechin was isolated from gambier through ethylacatate fraction by using chloroform and methanol (4 : 1) as eluent. The antibacterial activity test of (+)-catechin and water extract of gambier were carried out by using method of microdillution against 5 of gram positive bacteria, namely, Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus mutans, Streptococcus viridans and Basillus subtillis. For the detection of the mechanism of bacterial celluler destruction, the leakage of protein and nucleic acid were observed using UV visible spectrophotometer . In addition the leakage of K+ and Ca2+ ion was observed by using Atomic Absorption Spectrom\photometer (AAS) . Meanwhile the cellular morphological change were observed using scanning electron microscopy (SEM). Result showed that the minimum inhibition concentration (MIC) of (+)-catechin against Staphylococcus epidermidis, Streptococcus mutans, and Streptococcus viridans was 5.5 mg/ml, 8 mg/ml and 8 mg/ml respectively. While against , Staphylococcus aureus and Basillus subtillis the MIC was not obtained up to as high as 25 mg/ml. The MIC aqueous extract gambier against the Staphylococcus epidermidis was 22.5 mg/ml. The MIC was not obtained against the other four bacteria until the concentration 25 mg/ml. the leakage of protein, nucleic acid as well as K+ and Ca2+ ions were observed at 1 MIC and 2 MIC. The result SEM assay, it was found that the (+)-catechin and gambier extract causes plasma cell membrane damage and coagulation of the nucleoid by (+)-catechin worse than gambier extract. Key words: (+)-catechin, gambier, antibacterial, gram positive bacteria.
Description: This research has the objective of comparing the antibacterial activity as well as the mechanism of inhibition between (+)-catechin and gambier extract as the basis for choosing a substance to be developed as antimicrobial drug
URI: http://repository.uinjkt.ac.id/dspace/handle/123456789/48778
Appears in Collections:Prosiding Workshop/Lokakarya/Seminar



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