Please use this identifier to cite or link to this item: http://repository.uinjkt.ac.id/dspace/handle/123456789/47573
Title: Activity Test and Inhibitory Mechanism of Essential Oil of Lime Leaf (Citrus Aurantifolia, Swingle) Against Some of Bacterial Pathogens
Other Titles: Proceedings of the International Conference on Science and Technology (ICOSAT 2017)
Authors: Muhammad Yanis Musdja
Andria Agusta
Arys Medta Pariwidjayanti
Keywords: INTERNATIONAL PROCEEDING;https://doi.org/10.2991/icosat-17.2018.11;International Conference on Science and Technology (ICOSAT 2017);Advances in Intelligent Systems Research (AISR), volume 149
Issue Date: 10-Aug-2017
Publisher: Atlantis Press
Series/Report no.: Advances in Intelligent Systems Research (AISR), volume 149;https://doi.org/10.2991/icosat-17.2018.11
Abstract: Abstract Lime fruit (Citrus aurantifolia Swingle) is traditionally known to be useful for the treatment of various infectious diseases like fever, cough, toothache, sore throat, diphtheria, body odor, ringworm etc as folk medicine. Objectives: This study aims to determine the activity and mechanism of inhibition of the growth of some bacterial pathogens by the essential oil of lime leaves (Citrus aurantifolia, swingle). Materials and Methods: The lime leaves was obtained from Balitro Bagor, distilled by steam distillation process. The essential oil of lime leaf that obtained was analyzed by Gas Chromatography-Mass Spectroscopy. The activity test of anti-bacterial of essential oil of lime leaves was carried out by using the method mikrodilution against the bacterium Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Micrococcus luteus and Bacillus subtilis. For detect the mechanism of cell damage process of bacteria was observed leakage of proteins and nucleic acids by using Ultra Violet-Visible spectrophotometer and the leakage of cations K+ and Ca2+ was observed by using Atomic Absosrtion Spectrum. While bacterial cell morphology change was observed by using Scanning Electron Microscope (SEM). Results and Conclusion: From the research was obtained, Lime leaf contains about 0.4% (v/w) essential oils with the main components are geranial (10.39%), limonene (10.2%), neral (8.94%), kariofilena (5.72%) and citronellal (5.41%). The essential oil of lime leaf has the highest antibacterial activity on the bacteria tested was on Bacillus subtilis, with a minimum inhibitory concentration (MIC) of 0.125% (v/v). Test results nucleic acid and protein leakage and cations leakage of K+ and Ca2+ greatly increased from 1 MIC to 2 MIC concentration of the tested bacteria compared with the normal controls. While the observations that was detected by using Scanning Electron Microscope (SEM) showed worse cell damage by giving the higher doses to bacteria. Keyword: Essential oil of lime leaf, Antibacterial, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Micrococcus luteus, Bacillus subtilis
Description: This study aims to determine the activity and mechanism of inhibition of the growth of some bacterial pathogens by the essential oil of lime leaves (Citrus aurantifolia, swingle)
metadata.dc.description.uri: https://www.atlantis-press.com/search?q=Musdja.
URI: http://repository.uinjkt.ac.id/dspace/handle/123456789/47573
ISBN: ISBN: 978-94-6252-510-8, ISSN: 1951-6851
Appears in Collections:Prosiding Workshop/Lokakarya/Seminar



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